mgcl2 (New England Biolabs)
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New England Biolabs
mgcl2
Mgcl2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mgcl2/Magnesium+Chloride+(MgCl2)+Solution/pm41930845-53-5-9
Average 96 stars, based on 567 article reviews
Mgcl2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 567 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mgcl2/Magnesium+Chloride+(MgCl2)+Solution/pm41930845-53-5-9
Average 96 stars, based on 567 article reviews
mgcl2 - by Bioz Stars,
2026-11
96/100 stars
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Purification:Article Title: Dual-lock gated DNAzyme-CRISPR cascade for amplification-free ultrasensitive profiling of FTO demethylase activity. Article Snippet: N6-methyladenosine, the most prevalent internal mRNA modification in eukaryotes, dynamically regulates transcriptomic homeostasis via FTO-mediated demethylation.. CRISPR-based FTO detection technologies represent a significant research direction.. However, most current strategies depend critically on nucleic acid preamplification steps, which introduce substantial risks of amplification leakage, thereby compromising their ability to achieve stable, high-sensitivity FTO detection. Incubation:Article Title: A novel tripod probe and lateral flow test to improve CRISPR/Cas12a assay: benefits of branched probe based on trebler phosphoramidite modification. Article Snippet: CRISPR/Cas12a-based assays, when integrated with lateral flow tests (LFTs), provide highly specific nucleic acid detection in a simple, rapid, and equipment-free format.. Nevertheless, traditional DNA probes utilized for cleavage by Cas12a have limitations as the cleaved probe only has one label.. To overcome this challenge, we engineered a novel type of DNA probe with multiple fluorescein (FAM) labels and a biotin-labeled single-stranded DNA fragment (polyFAM probe). Article Title: ChromSMF: integrated profiling of histone modifications, protein-DNA interactions and DNA methylation on multi-kilobase DNA molecules Article Snippet: In summary, naked genomic DNA (gDNA) was extracted from Drosophila S2 cells using the Quick-DNA HMW MagBead Kit (Zymo, D6060) and fragmented to 6 kb using g-Tubes (Covaris, 520079) following the manufacturer’s instructions. gDNA was then in vitro methylated using mA and mC MTases individually (sample 3 and sample 4) or in combination (sample 7). .. Fully methylated CpG and GpC gDNA (sample 4) was generated by two consecutive 30 min incubations at 37°C with 8 U/μg DNA of M.CviPI GpC methyltransferase (in-house production) supplemented with 1.2 mM SAM (NEB, B9003S), followed by one 30 min incubation at 37°C with 16 U/μg DNA of M.SssI CpG methyltransferase (NEB, M0226L) in the presence of 7.5 μM Electro Mobility Shift Assay:Article Title: Cas9-Leveraged Single-Molecule Characterization of Sparse Plasmid Vectors in Heterogenous DNA Samples. Article Snippet: All in vitro digestion experiments were done with S. pyogenes Cas9 (NEB M0386S) in NEBuffer r3.1, if nothing else was specified. .. Electromobility shift assay (EMSA) was performed with dCas9 (NEB EnGen® Spy dCas9 (SNAP-tag®)) in NEBuffer r3.1 adjusted to 1 mM Polymerase Chain Reaction:Article Title: Targeted mutagenesis in Ehrlichia canis deleting the phage head-to-tail connector protein gene and its assessment as a vaccine candidate preventing canine ehrlichiosis. Article Snippet: Ehrlichia canis is primarily a Rhipicephalus sanguineus tick-borne rickettsial pathogen initially identified as causing canine monocytic ehrlichiosis, and human infections have also been reported in Venezuela, Mexico, and parts of Europe.. It is of high importance to have a vaccine suitable in protecting the canine host, which will aid in lessening E. canis infections also in humans.. Gene inactivation mutations in the phage head-to-tail connector protein genes (phtcp) from E. chaffeensis and A. marginale caused attenuated growth, and prior infection with the mutated bacteria induced protective immunity against wild-type bacterial infections in the natural hosts, independent of infection acquisition from blood-borne infection or tick-transmission. Mutagenesis:Article Title: Targeted mutagenesis in Ehrlichia canis deleting the phage head-to-tail connector protein gene and its assessment as a vaccine candidate preventing canine ehrlichiosis. Article Snippet: Ehrlichia canis is primarily a Rhipicephalus sanguineus tick-borne rickettsial pathogen initially identified as causing canine monocytic ehrlichiosis, and human infections have also been reported in Venezuela, Mexico, and parts of Europe.. It is of high importance to have a vaccine suitable in protecting the canine host, which will aid in lessening E. canis infections also in humans.. Gene inactivation mutations in the phage head-to-tail connector protein genes (phtcp) from E. chaffeensis and A. marginale caused attenuated growth, and prior infection with the mutated bacteria induced protective immunity against wild-type bacterial infections in the natural hosts, independent of infection acquisition from blood-borne infection or tick-transmission. Concentration Assay:Article Title: Amplification-free cancer diagnosis based on inhibition of Cas12a activity by site-specific 5mC-modified cfDNA Article Snippet: .. A concentration gradient of Methylation:Article Title: ChromSMF: integrated profiling of histone modifications, protein-DNA interactions and DNA methylation on multi-kilobase DNA molecules Article Snippet: In summary, naked genomic DNA (gDNA) was extracted from Drosophila S2 cells using the Quick-DNA HMW MagBead Kit (Zymo, D6060) and fragmented to 6 kb using g-Tubes (Covaris, 520079) following the manufacturer’s instructions. gDNA was then in vitro methylated using mA and mC MTases individually (sample 3 and sample 4) or in combination (sample 7). .. Fully methylated CpG and GpC gDNA (sample 4) was generated by two consecutive 30 min incubations at 37°C with 8 U/μg DNA of M.CviPI GpC methyltransferase (in-house production) supplemented with 1.2 mM SAM (NEB, B9003S), followed by one 30 min incubation at 37°C with 16 U/μg DNA of M.SssI CpG methyltransferase (NEB, M0226L) in the presence of 7.5 μM Generated:Article Title: ChromSMF: integrated profiling of histone modifications, protein-DNA interactions and DNA methylation on multi-kilobase DNA molecules Article Snippet: In summary, naked genomic DNA (gDNA) was extracted from Drosophila S2 cells using the Quick-DNA HMW MagBead Kit (Zymo, D6060) and fragmented to 6 kb using g-Tubes (Covaris, 520079) following the manufacturer’s instructions. gDNA was then in vitro methylated using mA and mC MTases individually (sample 3 and sample 4) or in combination (sample 7). .. Fully methylated CpG and GpC gDNA (sample 4) was generated by two consecutive 30 min incubations at 37°C with 8 U/μg DNA of M.CviPI GpC methyltransferase (in-house production) supplemented with 1.2 mM SAM (NEB, B9003S), followed by one 30 min incubation at 37°C with 16 U/μg DNA of M.SssI CpG methyltransferase (NEB, M0226L) in the presence of 7.5 μM |